Publications
Selected Publications
Kato K, Isbell HM, Fressart V, Denjoy I, Debbiche A, Itoh H, Poinsot J, George AL Jr, Coulombe A, Shea MA, Guicheney P. (2022) Novel CALM3 Variant Causing Calmodulinopathy With Variable Expressivity in a 4-Generation Family. Circ. Arrhythm. Electrophysiol. 2022 Feb 28:CIRCEP121010572. doi: 10.1161/CIRCEP.121.010572. Epub PMID: 35225649.
Mahling, R., Hovey, L., Isbell, H.M., Marx, D.C., Miller, M.S., Kilpatrick, A.M., Weaver, L.D., Yoder, J.B., Kim, E.H., Andresen, C.N.J., Li, S. and Shea, M.A. (2021) NaV1.2 EFL Domain Allosterically Enhances Reversal of Sequential Ca2+ Binding to WT and Pathogenic Calmodulin Mutants Bound to the Channel CTD Structure 29: 1-18.
PMID: 33770503 DOI: 10.1016/j.str.2021.03.002 (PDB ID: 6BUT; BMRB: 27740, 27745)
Editor’s Pick Highlight: Navigating the intricacies of cellular machinery (2021) Nathan, S. & Gabelli, S.B. Journal of Biological Chemistry (JBC) 296: 100832
Mahling, R., Rahlf, C.R., Hansen, S.C., Hayden, M.R., Shea, M.A. (2021) Ca2+-Saturated Calmodulin Binds Tightly to the N-Terminal Domain of A-Type Fibroblast Growth Factor Homologous Factors Journal of Biological Chemistry (JBC) 296: 100458. PMID: 33639159, DOI: 10.1016/j.jbc.2021.100458
Konstantinidis, K., Bezzeridis, V., Lai, L., Isbell, H.M., Wei, A., Wu, Y., Viswanathan, M.C., Blum, I.D., Granger, J.M., Heims-Waldron, D., Luczak, E.D., Murphy, K.R., Gratzk, D.H., Manta, B., Wang, Q., Kolodkin, A.L, Gladyshev, V.N., Hund, T.J., Pu, W., Wu, M.N., Cammarato, A., Bianchet, M.A., Shea, M.A., Levine, R.L., Anderson, M.E. (2020) MICAL1 operates a stereospecific sulfoxidation switch constraining CaMKII activation J. of Clinical Investigation 130:4663-4678.
Isbell, H.M., Kilpatrick, A. M., Lin, Z., Mahling, R. and Shea, M.A. (2018) Backbone resonance assignments of complexes of apo calmodulin bound to human voltage-dependent sodium channel IQ motif peptides from NaV1.1, NaV1.4 and NaV1.7. Biomolecular NMR Assignments , 12:283–289 BMRB: NaV1.1 (27190), NaV1.4 (27332), NaV1.7 (27260)
Marsden, A., Derry, S., Schneider, I., Scott, C., Westfall, T., Brastrom, L., Shea, M.A., Dawson, D.V., Slusarski, D.C. (2018) The Nkd EF-Hand Domain Modulates Divergent Wnt Signaling Outputs in Zebrafish Developmental Biology 434(1):63-73Fowler CA, Núñez Hernandez MF, O'Donnell SE, Yu L, Shea MA. (2017) Backbone and side-chain resonance assignments of (Ca2+)4-calmodulin bound to beta calcineurin A CaMBD peptide. Biomol NMR Assign. PMID: 28815458. DOI:10.1007/s12104-017-9762-7
Mahling R, Kilpatrick AM, Shea MA. (2017) Backbone resonance assignments of complexes of human voltage-dependent sodium channel NaV1.2 IQ motif peptide bound to apo calmodulin and to the C-domain fragment of apo calmodulin. Biomol NMR Assign. PMID: 28815458. DOI:10.1007/s12104-017-9762-7
*Hovey, L., *Fowler, C.A., Mahling, R., Lin, Z., Miller, M.S. Marx, D.C., Yoder, J.B., Kim, E.H., Tefft, K.M., Waite, B.C., Feldkamp, M.D., Yu, L., Shea, M.A. (2017) Calcium Triggers Reversal of Calmodulin on Nested Anti-Parallel Sties in the IQ Motif of the Neuronal Voltage-Dependent Sodium Channel NaV1.2 (PDB ID: 2M5E). Biophysical Chemistry 224:1-19 (http://dx.doi.org/10.1016/j.bpc.2017.02.006)
Feldkamp, M.D., Gakhar, L., Pandey, N. and Shea, M.A. (2015) Stuctural Insights into Molecular Specificity of Trifluoperazine Binding to Calcium-Saturated Calmodulin and the Role of Met144. Proteins: Structure, Function and Bioinformatics. PDB ID - 4RJD (http://dx.doi.org/10.1002/prot.24781)
Newman, R.A., Sorensen, B.R., Kilpatrick, A.M., Shea, M.A. (2014) Calcium-dependent energetics of calmodulin domain interactions with regulatory regions of the Ryanodine Receptor Type 1 (RyR1).
Biophys Chem. 193-194:35-49. [DOI: 10.1016/j.bpc.2014.07.004] (http://www.ncbi.nlm.nih.gov/pubmed/25145833)
Wang, X., Boyken, S.E., Hu, J., Xu, X., Rimer, R.P., Shea, M.A., Shaw, A.S., Andreotti, A.H., and Huang, Y.H. (2014) Calmodulin and PI(3,4,5)P3 cooperatively bind to the Itk pleckstrin homology domain to promote efficient calcium signaling and IL-17A production. Sci. Signal 7:337. [DOI: 10.1126/scisignal.2005147] (http://www.ncbi.nlm.nih.gov/pubmed/25097034)
Shea, M.A., Correia, J.J., Brenowitz, M.D. (2011) Introduction: Twenty Five Years of the Gibbs Conference on Biothermodynamics. Biophysical Chemistry 159:1-5. (http://dx.doi.org/10.1016/j.bpc.2011.07.002)
Evans, T.I.A., Hell, J.W., Shea, M.A. (2011) Thermodynamic Linkage between Calmodulin Domains Binding Calcium and Contiguous Sites in the C-Terminal Tail of Ca V1.2. Biophysical Chemistry 159:172-187. (http://dx.doi.org/10.1016/j.bpc.2011.06.007)
Feldkamp, M.D., Yu, L., Shea, M.A. (2011) Structural Determinants of Apo Calmodulin Binding to the IQ-Motif of the NaV1.2 Voltage-Dependent Sodium Channel. Structure 19:733-47.
O'Donnell, S.E., Yu, L., Fowler, C.A., Shea, M.A. (2011) Recognition of Beta-Calcineurin by the Domains of Calmodulin. Proteins: Structure, Function, Bioinformatics 79:765-786.
Feldkamp, M.D., O'Donnell, S.E., Yu, L., Shea, M.A. (2010) Allosteric Effects of the Anti-Psychotic Drug Trifluoperazine on the Energetics of Calcium Binding by the Calmodulin. Proteins: Structure, Function, Bioinformatics 78: 2265-2282 (http://dx.doi.org/10.1002/prot.22739)
O'Donnell, S. E., Newman, R.A.., Witt, T.J., Hultman, R., Froehlig, J., Christensen, A.P., Shea, M.A (2009) Thermodynamics and Conformational Change Governing Domain-Domain Interactions of Calmodulin. in Methods in Enzymology 466: 503-526 Edited by Ackers, G.K., Holt, J., Johnson, M.L. (http://dx.doi.org/10.1016/S0076-6879(09)66021-3)
Evans, T.I.A. and Shea, M.A. (2009) Energetics of Calmodulin Domain Interactions with the Calmodulin Binding Domain of CaMKII. Proteins: Structure, Function, Bioinformatics 76:47-61. (http://dx.doi.org/10.1002/prot.22317)
O'Donnell, S. E., Newman, R.A., Witt, T.J., Hultman, R., Froehlig, J., Christensen, A.P., Shea, M.A. (2009) Thermodynamics and Conformational Change Governing Domain-Domain Interactions of Calmodulin in Methods in Enzymology. 466: 503-526 (Johnson, M.L., Holt, J., Ackers, G.K., editors)
Erickson, J.R., Joiner, M.L., Guan X., Kutschke, W., Yang, J., Oddis, C.V., Bartlett, R.K., Lowe, J.S., O'Donnell, S., Aykin-Burns, N., Zimmerman, M.C., Zimmerman, K., Ham, A.L., Weiss, R.M., Spitz, D.R., Shea, M.A., Colbran, R.J., Mohler, P.J., Anderson, M. (2008) A Dynamic Pathway for Calcium-Independent Activation of CaMKII by Methionine Oxidation. Cell 133: 462-474 (http://dx.doi.org/10.1016/j.cell.2008.02.048)
Theoharis, N.T., Sorensen, B.R., Shea, M.A. (2008) Neuronal Voltage-Dependent Sodium Channel Type II (NaV1.2) IQ Motif Lowers Calcium Affinity of the C-domain of Calmodulin. Biochemistry 47: 112-123 (http://dx.doi.org/10.1021/bi7013129)
Newman, R.A., VanScyoc, W.S., Jaren, O.R., and Shea, M.A. (2008) Domain-Specific Interactions of Calmodulin Bound to Melittin Preferentially Increases Clacium Affinity of Sites I and II. Proteins: Structure, Function, Bioinformatics 71:1792-812. (http://dx.doi.org/10.1002/prot.21861)
Akyol, Z., Gakhar, L., Sorensen, B.R., Hell, J.W., and Shea, M.A. (2007) The NMDA Receptor NR1 C1 Region Bound to Calmodulin: Structural Insights into Functional Differences between Homologous Domains. Structure 15: 1603-1617 (http://dx.doi.org/10.1016/j.str.2007.10.012)
Li, Q., Cooper, J.J., Altwerger, G.H., Feldkamp, M.D., Shea, M.A., and Price, D.P. (2007) HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells. Nucleic Acids Research 35: 2503?2512. (http://dx.doi.org/10.1093/nar/gkm150)
Merrill, M.A., Malik, Z., Akyol, Z., Bartos, J.A., Leonard, A.S., Hudmon, A., Shea, M.A. and Hell, J.W. (2007) Displacement of a-Actinin from the NMDA Receptor NR1 C0 Domain by Ca2+/Calmodulin Promotes CaMKII Binding. Biochemistry 46:8485-97 (http://dx.doi.org/10.1021/bi0623025)
VanScyoc, W.S., Newman, R.A., Sorensen, B.R., Shea, M.A. (2006) Calcium Binding to Paramecium Calmodulin Mutants Having Domain?Specific Effects on Regulation of Ion Channels. Biochemistry 45: 14311-14324 (http://dx.doi.org/10.1021/bi061134d)
Ross J.B.A., Laws W., Shea M.A. (2006) Intrinsic Fluorescence in Protein Structure Analysis. in Protein Structures: Methods in Protein Structure and Structure Analysis: Luinescence Spectroscopy and Circular Dichroism, Eds. VN Uversky, EA Permyakov, Nova Science Publishers, Inc., New York. 55-72 ISBN 1600214045 (http://www.vanstockum.nl/product/7188809/Methods-In-Protein-Structure-And-Stability-Analysis.html)
Wang, B., Martin, S.R, Newman, R.A., Hamilton, S.L., Shea, M.A., Bayley, P.M., and Beckingham, K. (2004) Biochemical properties of V91G calmodulin: A calmodulin point mutation that deregulates muscle contraction in Drosophila. Protein Science 13: 3285-3297 (http://dx.doi.org/10.1110/ps.04928204)
Hines, R., Sorensen, B.R., Shea, M.A., Maury, W. (2004) PU.1 Binding to ets Motifs within the Equine Infectious Anemia Virus Long Terminal Repeat (LTR) Enhancer: Regulation of LTR Activity and Virus Replication in Macrophages. J. Virology 78: 3407?3418. (http://dx.doi.org/10.1128/JVI.78.7.3407-3418.2004)
Akyol, Z., Bartos, J., Jaren, O.R., Faga, L., Shea, M.A. and Hell, J.? (2004) Apo-Calmodulin Binds with its COOH-terminal Domain to the N-methyl-D-aspartate Receptor NR1 C0 Region. J. Biol. Chem. 279(3): 2166-2175. (http://dx.doi.org/10.1074/jbc.M302542200)
Faga, L.A., Sorensen, B.R., VanScyoc, W.S., and Shea, M.A. (2003) Basic Interdomain Boundary Residues in Calmodulin Decrease Calcium Affinity of Sites I and II by Stabilizing Helix-Helix Interactions. Proteins: Structure, Function & Genetics 50: 381-391. (http://dx.doi.org/10.1002/prot.10281)
Leonard, A.S., Bayer, K-U, Merrill, M., Shea, M.A., Schulman, H., and Hell, J.W. (2002) Regulation of CaMKII Docking to NMDA Receptors by Calcium/Calmodulin and a-Actinin. J. Biol. Chem. 277: 48441-48448 (http://dx.doi.org/10.1074/jbc.M205164200)
Jaren, O.R., Kranz, J.K., Sorensen, B.R., Wand, A.J., Shea, M.A. (2002) Calcium-Induced Conformational Switching of Paramecium Calmodulin: Changes in the Protein Backbone Observed by Heteronuclear NMR Studies. Biochemistry 41: 14158-14166. (http://dx.doi.org/10.1021/bi026340+)
VanScyoc, W.S., Sorensen, B.R., Rusinova, E., Laws, W., Ross, J.B.A., and Shea, M.A. (2002) Domain-Specific Fluorescence of Calmodulin: Phenylalanine Reports Exclusively on Calcium Binding to the N-Domain. Biophysical Journal 83: 2767-2790. (http://dx.doi.org/10.1529/biophysj.102)
Xiong, L-W, Newman, R.A., Rodney, G.C., Thomas, O., Zhang, J-Z, Persechini, A., Shea, M.A., and Hamilton, S.L. (2002) Lobe Dependent Regulation of Ryanodine Receptor Type One by Calmodulin. J. Biol. Chem. 277:40862-40870. (http://dx.doi.org/10.1074/jbc.M206763200)
Sorensen, B.R., Faga, L.A., Hultman, R., Shea, M.A. (2002) Interdomain Linker Increases Thermostability and Decreases Calcium Affinity of Calmodulin N-Domain. Biochemistry 41: 15-20. (http://dx.doi.org/10.1021/bi011718+)
Sun, H., Yin, D., Coffeen, L.A., Shea, M.A., and Squier, T.C. (2001) Mutation of Tyr138 Disrupts the Structural Coupling Between the Opposing Domains in Vertebrate Calmodulin. Biochemistry 40: 9605-9617. (http://dx.doi.org/10.1021/bi0104266)
VanScyoc, W. and Shea, M.A. (2001) Phenylalanine fluorescence studies of calcium binding to N-domain fragments of Paramecium calmodulin mutants show increased calcium affinity correlates with increased disorder. Protein Science 10:1758-1768. (http://dx.doi.org/10.1110/ps.11601)
Sorensen, B.R., Eppel, J.T., Shea, and M.A. (2001) Paramecium Calmodulin Mutants Defective in Ion Channel Regulation Associate with Melittin in the Absence of Calcium but Require it for Tertiary Collapse. Biochemistry 40:896-903. (http://dx.doi.org/10.1021/bi0023091)